Coding

Part:BBa_K4188032:Design

Designed by: Tobias Bröker   Group: iGEM22_WWU_Muenster   (2022-09-30)


PptAPS fused to PTS1 tag for the peroxisomal expression


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 787
    Illegal PstI site found at 1040
    Illegal PstI site found at 1360
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 787
    Illegal PstI site found at 1040
    Illegal PstI site found at 1360
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 787
    Illegal BamHI site found at 1059
    Illegal XhoI site found at 834
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 787
    Illegal PstI site found at 1040
    Illegal PstI site found at 1360
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 787
    Illegal PstI site found at 1040
    Illegal PstI site found at 1360
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

N/A


Source

N/A

References